{
  "paper_doi": "10.3389/fmars.2024.1361452",
  "bioproject": "PRJNA1049584",
  "run": {
    "run_accession": "SRR27126345",
    "sample_alias": "V4_KH-20-9-085",
    "library_strategy": "AMPLICON",
    "fastq_ftp": "ftp.sra.ebi.ac.uk/vol1/fastq/SRR271/045/SRR27126345/SRR27126345_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR271/045/SRR27126345/SRR27126345_2.fastq.gz",
    "fastq_bytes": "5455833;6335739"
  },
  "source_url": "https://ftp.sra.ebi.ac.uk/vol1/fastq/SRR271/045/SRR27126345/SRR27126345_1.fastq.gz",
  "source_sha256": "e1e891da131e070784624caab00553ec33349ad10dbb83fba78bdf1fbb8417cb",
  "total_forward_reads": 38293,
  "quality_passed_reads": 24879,
  "unique_quality_passed_reads": 10541,
  "selection": "10 most frequent distinct forward read sequences among reads of length >=250, ACGT only, expected errors <=1; ties sorted lexicographically",
  "processing": "No trimming, merging, denoising, chimera removal or taxonomic filtering. These are raw-read-derived demonstration sequences, NOT the published ASVs.",
  "records": [
    {
      "fasta_id": "Wu2024_V4_read_01",
      "run": "SRR27126345",
      "sample": "V4_KH-20-9-085",
      "original_read_id": "SRR27126345.476 M06504:14:000000000-JY3D5:1:1101:3211:12331/1",
      "length": "300",
      "quality_filtered_duplicate_count": "138",
      "representative_read_expected_errors": "0.1443437934717423"
    },
    {
      "fasta_id": "Wu2024_V4_read_02",
      "run": "SRR27126345",
      "sample": "V4_KH-20-9-085",
      "original_read_id": "SRR27126345.394 M06504:14:000000000-JY3D5:1:1101:25436:10653/1",
      "length": "300",
      "quality_filtered_duplicate_count": "121",
      "representative_read_expected_errors": "0.3797777855144483"
    },
    {
      "fasta_id": "Wu2024_V4_read_03",
      "run": "SRR27126345",
      "sample": "V4_KH-20-9-085",
      "original_read_id": "SRR27126345.1234 M06504:14:000000000-JY3D5:1:1102:23920:7587/1",
      "length": "300",
      "quality_filtered_duplicate_count": "108",
      "representative_read_expected_errors": "0.08295465738270218"
    },
    {
      "fasta_id": "Wu2024_V4_read_04",
      "run": "SRR27126345",
      "sample": "V4_KH-20-9-085",
      "original_read_id": "SRR27126345.1722 M06504:14:000000000-JY3D5:1:1102:10931:17425/1",
      "length": "301",
      "quality_filtered_duplicate_count": "105",
      "representative_read_expected_errors": "0.11936023807804332"
    },
    {
      "fasta_id": "Wu2024_V4_read_05",
      "run": "SRR27126345",
      "sample": "V4_KH-20-9-085",
      "original_read_id": "SRR27126345.34 M06504:14:000000000-JY3D5:1:1101:9315:2886/1",
      "length": "301",
      "quality_filtered_duplicate_count": "105",
      "representative_read_expected_errors": "0.1046243813896498"
    },
    {
      "fasta_id": "Wu2024_V4_read_06",
      "run": "SRR27126345",
      "sample": "V4_KH-20-9-085",
      "original_read_id": "SRR27126345.85 M06504:14:000000000-JY3D5:1:1101:5328:4199/1",
      "length": "300",
      "quality_filtered_duplicate_count": "103",
      "representative_read_expected_errors": "0.39611204357931856"
    },
    {
      "fasta_id": "Wu2024_V4_read_07",
      "run": "SRR27126345",
      "sample": "V4_KH-20-9-085",
      "original_read_id": "SRR27126345.1114 M06504:14:000000000-JY3D5:1:1102:23586:4495/1",
      "length": "300",
      "quality_filtered_duplicate_count": "103",
      "representative_read_expected_errors": "0.10686196407700711"
    },
    {
      "fasta_id": "Wu2024_V4_read_08",
      "run": "SRR27126345",
      "sample": "V4_KH-20-9-085",
      "original_read_id": "SRR27126345.1457 M06504:14:000000000-JY3D5:1:1102:27790:12372/1",
      "length": "300",
      "quality_filtered_duplicate_count": "101",
      "representative_read_expected_errors": "0.3133703994577293"
    },
    {
      "fasta_id": "Wu2024_V4_read_09",
      "run": "SRR27126345",
      "sample": "V4_KH-20-9-085",
      "original_read_id": "SRR27126345.224 M06504:14:000000000-JY3D5:1:1101:6685:7326/1",
      "length": "300",
      "quality_filtered_duplicate_count": "95",
      "representative_read_expected_errors": "0.11296331677604927"
    },
    {
      "fasta_id": "Wu2024_V4_read_10",
      "run": "SRR27126345",
      "sample": "V4_KH-20-9-085",
      "original_read_id": "SRR27126345.171 M06504:14:000000000-JY3D5:1:1101:8437:6247/1",
      "length": "300",
      "quality_filtered_duplicate_count": "92",
      "representative_read_expected_errors": "0.12518600613139716"
    }
  ],
  "description": "Wu et al. (2024) environmental V4 forward reads selected from public FASTQ; not published ASVs."
}