Tree Identification (Mitochondrial genes)

Status

Ready.

(1) Anonymous sequences    Examples: Fish 12S (≈ 4 secs) (Yu et al. 2022) Corals 12S (≈ 5 secs) (Shinzato et al. 2021) Copepods COI (≈ 50 secs) (Hirai et al. 2022)

>OTU_006_B06-0m CACCGCGGTTATACGATAGGCCCAAGTTGACAGACCCCGGCGTAAAGCGTGGTTAGGGAAAACTCAAAACTAAAGCCGAATATCTTCAGGGCAGTTATACGCTTCCGAAGACACGAAGCCCTTCCACGAAAGTGACTTTATTACCCCCGACCCCACGAAAGCTAGGACACAAA >OTU_321_B06-0m GCCACCGCGGTTATACGAGAGACCCTAGTTGATTGAAGCGGCGTAAAGAGTGGTTATGGAATTTTCTACCCTAAAGCAGAAAACCTCTCAAACTGTTATACGCACCCAGAGGTTGAAACCCCTTACACGAAAGTGACTTTATTTTCGCCTACCAGAAGCCACGAAAGCTAGGACACAAA >OTU_007_B06-0m GCCACCGCGGTTATACGAGGGACCCTAGTTGATTTAATCGGCGTAAAGAGTGGTTATGGAGAATAAGAAACTAAAGCCGAAGACCTCTTAGGCCGTCATACGTACCTAGAGGCTCGAATAACAAACACGAAAGTAGCTTTACCCCTTCCTGCCAGAACCCACGAGAGCTGGGATACAAA >OTU_032_B06-0m GCCACCGCGGTTATACGAGAGACCCTAGTTGATTGAAGCGGCGTAAAGAGTGGTTATGGAATTTTCTACCCTAAAGCAGAAAACCTCTCAAACTGTTATACGCACCCAGAGGTTGAAACCCCTTACACGAAAGTGACTTTATTTTCGCCTACCAGAAGCCACGAAAGCTGGGACACAAA >OTU_118_B06-0m GCCACCGCGGTTATACGAGAGGCCCAAGTTGATGACCACCGGCGTAAAGAGTGGTTACGGAAGCACTTCACTAAAGCCGAACAACCTCTAGGCTGTCATACGCACCTGAGGCTACGAAGCTCCCCCACGAAAGTGGCTTTACCCCTCCCTGAACCCACGACAGCTATGATACAAA >OTU_324_B06-0m ACCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCC >OTU_347_B06-0m CACCGCGGTCATACGATTGACCCAAATTAATAGACACCCGGCGTAAAGAGTGTCAAAGAACAATGTAAAAATAAAGTCAAACCTTAATTAAGCTGTAAAAAGCCATAATTAAAATTAAGTTAAACTACGAAAGTAACTTTACCATAAACTGAGTACACGACAACTAAGACCCAAA >OTU_023_B06-0m AGCCACAGCGGTTATACGATAGGCCCAAGTTGACAGACCCCGGCGTAAAGCGTGGTTAGGGAAAACTCAAAACTAAAGCCGAATATCTTCAGGGCAGTTATACGCTTCCGAAGACACGAAGCCCTTCCACGAAAGTGACTTTATTACCCCCGACCCCACGAAAGCTAGGACACAAA >OTU_031_B06-0m CCACCGAGGTTATACGATAGGCCCAAGTTGACAGACCCCGGCGTAAAGCGTGGTTAGGGAAAACTCAAAACTAAAGCCGAATATCTTCAGGGCAGTTATACGCTTCCGAAGACACGAAGCCCTTCCACGAAAGTGACTTTATTACCCCCGACCCCACGAAAGCTAGGACACAAA >OTU_391_B06-0m GCCACCGCGGTTATACGAGAGGCCCAAGTTGACAGACACCGGCGTAAAGCGTGGTTAAGGAAAACTTAGACTAAAGCCGAACACCTTCAAGGCAGTTATACGCATCCGAAGATACGAAGCCCCACCACGAAAGTGGCTTTATAACCCCTGACCCCACGAAAGCTATGATACAAA >OTU_040_B06-0m CCACCGAGGTTATACGAGGGACCCTAGTTGATTTAATCGGCGTAAAGAGTGGTTATGGAGAATAAGAAACTAAAGCCGAAGACCTCTTAGGCCGTCATACGTACCTAGAGGCTCGAATAACAAACACGAAAGTAGCTTTACCCCTTCCTGCCAGAACCCACGAGAGCTGGGATACAAA >OTU_157_B06-0m CAGCCAACGCGGTTATACGAGAGACCCTAGTTGATTGAAGCGGCGTAAAGAGTGGTTATGGAATTTTCTACCCTAAAGCAGAAAACCTCTCAAACTGTTATACGCACCCAGAGGTTGAAACCCCTTACACGAAAGTGACTTTATTTTCGCCTACCAGAAGCCACGAAAGCTGGGACACAAA >OTU_003_C00-300m CACCGCGGTCAAACGAGCTAGTGCCCGAGCAGATAACTAACGGCGTAAAGAGTGGTTAGGGAAACCCAAAACTAAAGCTGAACGCCCGCAGGGCTGTGATACGCATCCGACGGTACGAAACCCCACCACGAAAGTGACTTTAACCAACCCGAACCCACGATAGCCAGGATA >OTU_005_C00-300m CACCGCGGTTATACGATAGGCCCAAGTTGACAGACCCCGGCGTAAAGCGTGGTTAGGGAAAACTCAAAACTAAAGCCGAATATCTTCAGGGCAGTTATACGCTTCCGAAGACACGAAGCCCTTCCACGAAAGTGACTTTATTACCCCCGACCCCACGAAAGCTAGGACA >OTU_021_C00-300m CACCGCGGTTATACGAGAGACCCAAGTTGATAAAAAACGGCGTAAAGCGTGGTTAAGAAAATTAAACTATAGGGTTGAATGGCCTCAATTTAGTTAAATGCACTCGAAGCCGTGAAGCACAATTACGAAAGTAGCCCTACCAAATCTCTGACTCCACGAAAGCCATAAAA >OTU_085_C00-300m CACCGCGGTCAAACGAGATTGTGCCCAAGCGGATAACCAACGGCGTAAAGAGTGGTTAGGGAGACCCCCCAACTAAAGCTGAACACCCACAGAGCCGTGATACGCATCCGACGGCATGAAACCCCACCACGAAAGTGGCTTTAACCCAGCCCGAACCCACGACAGCTAGGACA >OTU_004_C00-300m CACCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACC >OTU_092_C00-300m AGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTATGTAGGCGGATCAAAAAGTTAGATGTGAAATCCCTGGGCTCAACCCAGGAACTGCATTTAAAACTAGTGATCTAGAATTTGGTAGGGGTTAGTAGAATTTCCAGTGTAGAGGTGAAATTCGTAGATATTGGAAAGAATACCAGTGGCGAAGGCGACTAACTGGGCCATTTTTGACGCTGAGATACGAAAGCGTGGGTAG >OTU_1127_C00-300m AGCCGCGGTAATACAGAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTATGTAGGCGGATCAAAAAGTTAGATGTGAAATCCCTGGGCTCAACCCAGGAACTGCATTTAAAACTAATGATCTAGAATTTGGTAGAGGTCAGTAGAATTTCCAGTGTGGAGGTGAAATTCGTAGATATTGGAAAGAATACCGGTGGCGAAGGCGACTGACTGGGCCATTTTTGACGCTGAGATACGAAAGCGTGGGTAG >OTU_741_C00-300m CACCGCGGTCAAACGAGCTAGTGCCCGAGCAGATAACTAACGGCGTAAAGAGTGGTTAGGGAAACCCAAAACTAAAGCTGAACGCCCGCAGGGCTGTGATACGCATCCGACGGTACGAAACCCCACCACGAAAGTGGCTTTAACCCAGCCCGAACCCACGACAGCTAGGACA or upload file

Number of queries	Not selected: Reconstructs tree with all anonymous sequences First 1 anonymous sequence First 2 anonymous sequences First 3 anonymous sequences First 4 anonymous sequences First 5 anonymous sequences First 6 anonymous sequences First 7 anonymous sequences First 8 anonymous sequences First 9 anonymous sequences First 10 anonymous sequences
(2) Databases
Anonymous DB (uploaded sequences) -num_alignments: # of hits for each query sequence 0 hits: not used 1 hits 2 hits 3 hits 4 hits 5 hits 10 hits 15 hits 20 hits 30 hits 40 hits 50 hits 60 hits 70 hits 80 hits 90 hits 100 hits 110 hits 120 hits 130 hits 140 hits 150 hits -evalue: E-value threshold for reported sequences 1e-5: narrow 1e-4 1e-3 1e-2 1e-1 1: wide
Pre-installed DB MIDORI2: (mtDNA 15 gene sequences) GenBank265 [May. 2025] -num_alignments: Species DB (MIDORI2, longest) 0: not used 1 hits 2 hits 3 hits 4 hits 5 hits 10 hits 15 hits 20 hits 30 hits 40 hits 50 hits 60 hits 70 hits 80 hits 90 hits 100 hits 110 hits 120 hits 130 hits 140 hits 150 hits Haplotype DB (MIDORI2, uniq) 0 hits 1 hits 2 hits 3 hits 4 hits 5 hits 10 hits 15 hits 20 hits 30 hits 40 hits 50 hits 100 hits 300 hits -evalue: 1e-5: narrow 1e-4 1e-3 1e-2 1e-1 1: wide
User DB Example: MiFish DB -num_alignments: 0: not used 1 hits 2 hits 3 hits 4 hits 5 hits 10 hits 15 hits 20 hits 30 hits 40 hits 50 hits 100 hits -evalue: 1e-5: narrow 1e-4 1e-3 1e-2 1e-1 1: wide
(3) Measure of Confidence
Bootstrap analysis: Branch confidences in a phylogenetic tree	No 100 times

Sequence Extraction