SILVA LSU: Parc / Ref NR99 comparison

Server: yurai (CGI)

Tree Identification (Fungi) · Sequence Extraction (Fungi)

5 October 2026 · Public beta testing

Same 27 decaying-wood eDNA OTUs (Shirouzu et al. 2020, Figs. 2 and 3 ; Matsuoka 2022, p. 221, Fig. 3). Tree settings: first 10 queries; 20 reference hits per query; up to 150 anonymous hits; E-value 1e-3; minimum 80% identity and 70% query coverage; MAFFT, trimAl, NJ TN93, 100 bootstrap replicates. The per-OTU table below separately searches all 27 sequences under the same BLAST settings.

Initial LSU database: Parc. For this example, Parc retains closer references for all 27 OTUs. This does not establish that Parc is preferable for every organism or marker region. Higher BLAST identity does not by itself establish species identity.

MeasureParcRef NR99
Reference sequences131252195279
Queries with qualifying hits2727
Best-hit identity range (%)94.613–100.00085.668–95.410
Tree tips145103
Retained alignment sites258305
Tree pipeline seconds (single stage run; not a benchmark)12.84.89
Extraction keyword Dacrymycetes: matching records1911

Taxonomy is retained from SILVA. Some lineages disagree with submitted organism names: LC003982.1.611 is placed in Agaricomycetes / Serpulaceae / Serpula, but is named “uncultured Dacrymycetes”. MH877696.1.855 is placed in Tremellomycetes / Tremellales, but is named “Ditiola nuda”. The extraction counts above are literal keyword matches, not independently verified class membership. Missing family ranks are shown as unclassified.

No example accessions were removed from SILVA. This is a comparison of database choices, not an independent validation. The source paper used RAxML; these NJ trees do not reproduce its inference method.

Parc

Open tree, alignment and downloads · Sequence extraction result

Parc tree

Ref NR99

Open tree, alignment and downloads · Sequence extraction result

Ref NR99 tree

All 27 OTUs: highest-bit-score qualifying hit

One hit is shown when scores tie; names below are database organism labels, not species assignments. Download comparison TSV

OTUParc organism labelIdentity / coverage (%)Ref NR99 organism labelIdentity / coverage (%)
OTU_3Dacrymyces australis100.0 / 100.0Dacrymyces stillatus95.238 / 86.897
OTU_29uncultured Dacrymycetes98.969 / 100.0Ramaria stricta85.668 / 100.0
OTU_96Dacrymycetes sp.99.31 / 98.639Trechispora farinacea86.557 / 100.0
OTU_97Ditiola nuda97.241 / 100.0Dacrymyces stillatus92.063 / 86.897
OTU_103Dacrymyces lacrymalis100.0 / 96.552Dacrymyces stillatus92.46 / 86.897
OTU_120uncultured Dacrymycetes98.276 / 100.0Dacrymyces stillatus92.063 / 86.897
OTU_155Dacrymyces variisporus96.587 / 96.7Phaffia rhodozyma90.909 / 100.0
OTU_177Dacrymyces capitatus98.625 / 100.0Dacrymyces stillatus92.063 / 86.897
OTU_194uncultured Dacrymycetes98.621 / 100.0Dacrymyces stillatus92.46 / 86.897
OTU_196Dacrymyces variisporus100.0 / 96.7Phaffia rhodozyma88.312 / 100.0
OTU_211Dacrymyces minutus95.088 / 95.946Mrakia blollopis88.925 / 100.0
OTU_214Dacrymycetes sp. NBRC 110597100.0 / 96.026Botryobasidium subcoronatum94.426 / 100.0
OTU_228Dacryoscyphus chrysochilus98.233 / 97.586Dacrymyces stillatus90.476 / 86.897
OTU_241Dacrymycetes sp. 7 ST-2014100.0 / 96.04Phaffia rhodozyma91.558 / 100.0
OTU_248uncultured Dacrymycetes100.0 / 100.0Dacrymyces stillatus92.857 / 86.897
OTU_258uncultured Dacrymycetes99.661 / 100.0Ramaria stricta88.026 / 100.0
OTU_263uncultured Dacrymycetes99.322 / 100.0Ramaria stricta87.013 / 100.0
OTU_266Ceratobasidium pseudocornigerum99.333 / 99.01Fomitopsis palustris95.41 / 100.0
OTU_272uncultured Dacrymycetes100.0 / 100.0Botryobasidium subcoronatum94.098 / 100.0
OTU_278Dacrymyces citrinus98.535 / 91.919Phellinus noxius OVT-YTM/9789.508 / 100.0
OTU_323Dacrymyces minutus95.122 / 95.96Trechispora farinacea89.508 / 100.0
OTU_345uncultured Dacrymycetes95.593 / 100.0Ramaria stricta87.948 / 100.0
OTU_356uncultured Dacrymycetes99.317 / 100.0Sarcomyxa serotina85.668 / 98.976
OTU_367Cerinosterus luteoalbus94.613 / 100.0Mrakia blollopis89.251 / 100.0
OTU_466uncultured Dacrymycetes97.627 / 100.0Ramaria stricta86.319 / 100.0
OTU_494uncultured Dacrymycetes100.0 / 100.0Dacrymyces stillatus93.651 / 86.897
OTU_495Femsjonia uniseptata100.0 / 100.0Mrakia blollopis88.925 / 100.0

SILVA 138.2 documentation · SILVA attribution and CC BY 4.0 license · Example source paper